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혈관동결후 시간 경과에 따른 평활근세포 및 내막세포 재생에 관한 연구
REGENERATION OF ENDOTHELIUM AND SMOOTH MUSCLE CELL OF RAT FEMORAL ARTERY AFTER FREEZING INJURY : HISTOLOGIC, IMMUNOHISTOCHEMICAL AND SCANNING ELECTRON MICROSCOPIC STUDY

대한구강악안면외과학회지 1998년 24권 1호 p.47 ~ 59

강주일, 이영지, 박철완,

소속 상세정보

강주일 (  ) - 단국대학교 치과대학 구강악안면외과학교실
이영지 (  ) - 단국대학교 치과대학 구강악안면외과학교실
박철완 (  ) - 단국대학교 치과대학 구강악안면외과학교실

KMID : 0355619980240010047

Abstract

결론
1. 혈관확장은 술후 30일까지 관찰되었고, 내막세포와 평활근세포는 5일째부터 증식되기
시작했다. 근육세포의 배열이 불규칙하였고, 내막의 두께는 큰 변화가 없었다. 중막 및 외막
의 염증세포 침윤은 동결 후 5일까지 증가하다가 점차 감소하였다.
2. 주사전자현미경적 소견에서는 3일째 소견에서 내막세포의 파괴 및 탈락이 보였고, 재생
되는 내막세포도 부분적으로 관찰되었다. 10일째에는 내막세포가 손상 인접부로 부터 혈관
동결부로 증식 이동하고 있었으며, 30일째에는 내막세포 재생에 의해 배열은 불규칙하였다.
3. 대조군에서는 동맥의 내막세포, 평활근세포 및 주위 간엽세포에서 PCNA 양성반응이
관찰되었고, 동맥의 평활근세포 및 주위 혈관에서 α - SMA 양성반응이 보였다. Vimentin
에는 동맥벽에 있는 내막세포, 평활근세포 그리고 주위 간엽조직에서 양성 반응이 나타났다
4. 동결후 10일째에 내막세포에서 PCNA 양성반응이 나타났으며, 20일째는 반응이 매우
활발하였다. 평활근세포와 간엽세포는 5일째 양성반응이 관찰되었으며, 20일째는 조직 병리
학적으로 거의 치유된 양상을 보였다.
5. α - SMA조직소견에서 평활근세포는 5일째부터 반응하여 30일째 거의 회복되었으며,
주위 혈관은 3일째 발현되었으며 20일째는 치유된 양상이었다.
6. Vimentin 반응에서 내막세포는 5일째부터 양성반응이 보이기 시작하였고, 20일째는 대
조군과 유사한 양상을 보였다. 평활근세포 및 주위 간엽세포는 5일째부터 양성반이이 나타
났으며, 시간이 경과할수록 그 반응이 증가하였다.
이상의 소견에서 대퇴혈관의 내막세포는 동결 손상 후 20일, 평활근세포는 10∼20일 사이,
혈관 주위 간엽세포들은 20일내에 재생치유되었다.
#초록#
Cryogen kills living cells and cryosurgery has been introduced int he clinical field to
destroy tumors such as hemangioma or vascular lesion. And recently, vascular freezing
technique has been suggested as a new physical method of relief of the vasospasm.
Although it is an advanced technique in treating some tumors or malignancy and
potential modality in relieving the vasospasm, the detailed healing after vascular injury
of cryogen of artery and the healing after cryodamage, especially focussed on the
change and regeneration of endothelial cell and smooth muscle cell.
We sacrificed the experimental animals by 3 days, 5 days, 10 days, 20days, 30 days
and observed the sequential change that occur during regeneration of femoral artery
using a histologic, immunohistochemical and SEM study after the vascular freezing.
The results were as follow :
1. Vasodilation was observed by 30 days and endothelial cell and smooth muscle cell
was beginning to increase from 5th day. The arrangement of muscle cell was irregular
and the thickness of intima did not largely change. Infiltration of inflammatory cell into
media & adventitia increased on 5th day, and gradually decreased later.
2. In the view of SEM, the endothelial cell was destructed and falling off, and partly
regenerated at 3 days. On 10th day, the endothelial cell was moving into damaged area
and repairing it. On 30th day, the endothelial cell was fully covered the damaged area,
but having a irregular arrangement observed.
3. In control group, PCNA positive reaction was observed in endothelium, smooth
muscle cell and surrounding mesenchymal cell of artery. Smooth muscle cell and
perivascular vessels showed α-SMA positive reaction, and endothelial cell of arterial
wall, smooth muscle cell and surrounding mesenchymal tissue of artery showed vimentin
positive reaction.
4. In the PCNA study, endothelial cell showed positively reaction on 10th day and
actively reacted on 20th day. Smooth muscle, perivascular mesenchymal cells were
beginning to react positively on 5th day, and almost histopahologically repaired on 20th
day.
5. In the α-SMA histologic view, smooth muscle cell was beginning to react from
5th day, and almost repaired on 30th day. Perivascular arteries were reacting on 3rd day
and repaired on 20th day.
6. In Vimentin-reaction view, endothelial cells were beginning to react positively from
5th day and showing a similar reaction of control goup. Smooth muscle cell, perivascular
mesenchymal cells were showing a positive reaction from 5th day, and then reacting
more positively.
Based on these findings, intima of artery was repaired at 20 days, smooth muscle cell
between 10 and 20 days, perivascular mesenchymal cells were repaired within 20 days
after cryodamage.